Direct and indirect gene replacements in Aspergillus nidulans.
نویسندگان
چکیده
منابع مشابه
Down-Regulation of sidB Gene by Use of RNA Interference in Aspergillus nidulans
Background: Introduction of the RNA interference (RNAi) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. In the filamentous fungus Aspergillus nidulans, the gene sidB plays an essential role in septation, conidiation and vegetative hyphal growth. In the present study, we benefited from the RNAi strate...
متن کاملAspergillus nidulans
Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...
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Mitosis in growing hyphae of haploid and diploid Aspergillus nidulans has been studied with the light microscope in Helly-fixed preparations. At the periphery of the nucleus a sharply defined granule is invariably found which has strong affinity for acid fuchsin. Mitosis begins with the duplication of this granule and the development inside the nucleus of a gradually lengthening fibre between t...
متن کاملNovel sexual-cycle-specific gene silencing in Aspergillus nidulans.
We report a novel sexual-cycle-specific gene-silencing system in the genetic model Aspergillus nidulans. Duplication of the mating type matA(HMG) gene in this haploid organism triggers Mat-induced silencing (MatIS) of both endogenous and transgenic matA genes, eliminates function of the encoded SRY structural ortholog, and results in formation of barren fruiting bodies. MatIS is spatiotemporall...
متن کاملAntisense silencing of the creA gene in Aspergillus nidulans.
Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, ...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1985
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.5.7.1714